Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Association between acetaminophen metabolites and CYP2E1 DNA methylation level in neonate cord blood in the Boston Birth Cohort Clinical Epigenetics Springer Nature Link What Is Acetaminophen? Definition, Uses, Side Effects, and Risks Olympic Behavioral Health Paracetamol (Acetaminophen) hepatotoxicity and its management. How to Fix Glutathione from Tyleon TikTok Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research