Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Frontiers Glutathione deficiency in the pathogenesis of SARS CoV 2 infection and its effects upon the host immune response in severe COVID 19 disease Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Acute acetaminophen paracetamol toxicity Paracetamol metabolism. Download Scientific Diagram Interleukin 4 deficiency protects mice from acetaminophen induced liver injury and inflammation by prevention of glutathione depletion Inflammation Research Springer Nature Link