Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. The mechanism of action of N acetylcysteine (NAC): The emerging role of H2S and sulfane sulfur species ScienceDirect Simplify Rx Hmm Concept cleared from Simplify Rx Instagram Acetaminophen Induced Hepatotoxicity: a Comprehensive Update Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Dr. Laura E. Riley joined @GoodMorningAmerica recently to clear up some lingering confusion about Tylenol (acetaminophen) use during pregnancy. A new study from the University of London found that acetaminophen is safe