Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Is Tylenol Safe During Pregnancy? Risk Factors, Not Causation Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram