Acetaminophen metabolism. Acetaminophen is metabolized primarily by Download Scientific Diagram Old and New Analgesic Acetaminophen: Pharmacological Mechanisms Compared with Non Steroidal Anti Inflammatory Drugs Tylenol's Impact on Glutathione and Liver Damage Acetaminophen (Paracetamol) Springer Nature Link Frontiers Analgesic Effect of Acetaminophen: A Review of Known and Novel Mechanisms of Action Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.