Association between acetaminophen metabolites and CYP2E1 DNA methylation level in neonate cord blood in the Boston Birth Cohort Clinical Epigenetics Springer Nature Link Why you should avoid REGULAR acetaminophen use for babies and pregnancy 3 Little Plums Taking Tylenol? The Glutathione Connection Dr. Green Mom Tylenol, Leucovorin, Glutathione, & Autism Since Tylenol is such a hot topic right now, did you know it depletes glutathione levels? When your body processes Tylenol in the liver, one of the pathways creates a toxic byproduct. To neutralize Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.