How Keeping Glutathione Levels Up Can Benefit Your Health & Your Vitamin D Status GrassrootsHealth Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. What to Avoid When Taking Glutathione Thornhill's Pharmacy Glutathione: The Guardian Of Hair Color And Health Sex differences in glutathione metabolism and acetaminophen toxicity Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: