Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione, Tylenol & Your Health: What You Should Know Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Acetaminophen causes neurodevelopmental injury in susceptible babies and children: no valid rationale for controversy