Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Your livers unsung hero: Glutathione , Lets talk about what really happens when you take Tylenol (acetaminophen)., Your liver breaks down acetaminophen through several pathways. One pathway creates Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors