Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol Glutathione Kizer Wellness LLC Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research Interaction with Ethanol Alcohol Metabolism Glutathione, Alcohol and drugs Acetaminophen causes neurodevelopmental injury in susceptible babies and children: no valid rationale for controversy